|
Status |
Public on Jul 01, 2007 |
Title |
Lymphoblastoid Cell Line for Individual 8116.4 log phase |
Sample type |
RNA |
|
|
Source name |
Lymphoblastoid_cell_line
|
Organism |
Homo sapiens |
Characteristics |
ped: 8116 id: 4 fatid: 1 motid: 2 sex: 1 country: UK DDAST: CONTROL rs3744350: -- rs16492: TT rs16235: AG rs16238: CC rs558317: -- rs498973: -- rs16495: CC rs12946701: -- rs16519: GG rs16522: TC rs544198: -- rs657672: CT rs657723: TC rs16527: TC rs3025203: GG rs801259: -- rs3744353: GG rs2301775: AA rs16531: CC rs521633: AA rs16530: CT rs486512: CC rs1106769: -- rs2061342: GG rs7214151: TT rs7208487: TT rs9906612: AA rs4795369: AG rs11655550: TC rs4611492: TC rs12450559: AG rs12453682: TC rs1877032: AG rs879606: GG rs9892427: -- rs1877031: TC rs2271308: GA rs931992: CC rs2941503: GA rs2941504: GA rs1565922: CT rs907092: GA rs9911688: CC rs9303277: CT rs2060941: -- rs9908694: CC rs11557467: -- rs8067378: AG rs2305480: -- rs2290400: AG rs7216389: TC rs3744246: CC rs4795405: CT rs8079416: TT rs4795408: GG rs1007654: -- rs3902025: CC rs3894194: CC rs3859192: CC rs921651: AG rs8066582: CC rs8080546: CC rs2071369: CC rs2302776: GG rs3934886: TT rs11078936: CC rs868150: CC rs7502966: CC rs1568400: AG rs939348: CC rs3744805: GG rs2071427: GG rs2269457: AA rs2071570: GG rs7211770: AA rs2280400: -- rs13706: GA rs2715553: TT rs4890109: GG rs2715556: GG rs2715555: CC rs13695: GG rs11650680: CC rs2015561: TT rs538399: TT rs7214466: TT rs8078319: CT rs749906: CT rs4890115: CC rs2290207: -- rs7214311: AG rs896136: TT rs9675320: TT rs2023906: AG rs3136685: GG rs608517: AG rs1358175: TC rs757411: TC rs11659024: AG rs1474454: CT rs1526603: CC rs2014704: CC rs2462961: CC rs2429553: GG rs1880832: AG rs9915334: TC rs9303295: AA rs1405334: CT rs4890126: AG rs4890127: CC rs2109217: CC rs8079180: GG rs2429542: AA rs975078: AA rs9898164: AA rs719867: CC rs981684: AA rs12453124: CC rs4132126: -- rs7209228: CC rs7219451: CC rs7211017: GG
|
Treatment protocol |
n/a
|
Growth protocol |
Previously transformed cryo-preserved EBV cell lines were grown as 500 ml roller cultures. Once log phase had been obtained, cells were pelleted, media discarded and a mixture of RLT buffer and β-mercaptoethanol added. Pellets were vortexed to ensure thorough re-suspension after which they were frozen at -70oC and kept at -80oC.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted in using the RNeasy Maxi Kit.
|
Label |
biotin
|
Label protocol |
Procedure used standard Affymetrix labelling protocol.
|
|
|
Hybridization protocol |
Procedure used standard Affymetrix labelling protocol.
|
Scan protocol |
Procedure used standard Affymetrix labelling protocol.
|
Description |
no additional
|
Data processing |
RMA Express
|
|
|
Submission date |
Jun 08, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Goncalo Abecasis |
E-mail(s) |
goncalo@umich.edu
|
Organization name |
University of Michigan
|
Street address |
1420 Washington Heights
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE8052 |
Genetic variants regulating ORMDL3 expression are determinants of susceptibility to childhood asthma |
|
Relations |
Reanalyzed by |
GSE64985 |
Reanalyzed by |
GSE119087 |