|
Status |
Public on Jul 01, 2007 |
Title |
Lymphoblastoid Cell Line for Individual 8113.3 log phase |
Sample type |
RNA |
|
|
Source name |
Lymphoblastoid_cell_line
|
Organism |
Homo sapiens |
Characteristics |
ped: 8113 id: 3 fatid: 1 motid: 2 sex: 1 country: UK DDAST: CASE rs3744350: CC rs16492: TT rs16235: AG rs16238: CA rs558317: CC rs498973: CC rs16495: CC rs12946701: AA rs16519: GG rs16522: TT rs544198: TT rs657672: CC rs657723: TT rs16527: TT rs3025203: GG rs801259: TT rs3744353: GG rs2301775: AA rs16531: TT rs521633: CC rs16530: CC rs486512: CC rs1106769: -- rs2061342: GG rs7214151: TT rs7208487: TT rs9906612: AA rs4795369: AA rs11655550: TC rs4611492: TT rs12450559: AA rs12453682: TT rs1877032: AA rs879606: GG rs9892427: TT rs1877031: TT rs2271308: GG rs931992: AA rs2941503: GG rs2941504: GG rs1565922: CC rs907092: GG rs9911688: CT rs9303277: CT rs2060941: GG rs9908694: CT rs11557467: GT rs8067378: AG rs2305480: CC rs2290400: AG rs7216389: TC rs3744246: CC rs4795405: CC rs8079416: CT rs4795408: AG rs1007654: GG rs3902025: AA rs3894194: TC rs3859192: TT rs921651: AA rs8066582: TT rs8080546: CC rs2071369: CC rs2302776: AG rs3934886: TT rs11078936: TT rs868150: TT rs7502966: TT rs1568400: AA rs939348: TT rs3744805: GG rs2071427: AG rs2269457: AG rs2071570: GT rs7211770: AG rs2280400: CC rs13706: GG rs2715553: CT rs4890109: -- rs2715556: GG rs2715555: CT rs13695: GA rs11650680: CC rs2015561: TC rs538399: TC rs7214466: TC rs8078319: CC rs749906: CC rs4890115: TC rs2290207: GG rs7214311: AA rs896136: TT rs9675320: TT rs2023906: AG rs3136685: GG rs608517: AA rs1358175: TT rs757411: TT rs11659024: GG rs1474454: TT rs1526603: TT rs2014704: CC rs2462961: CC rs2429553: AG rs1880832: AG rs9915334: TC rs9303295: AA rs1405334: CT rs4890126: GG rs4890127: CT rs2109217: CA rs8079180: GA rs2429542: AA rs975078: GG rs9898164: AG rs719867: CT rs981684: GG rs12453124: CT rs4132126: AG rs7209228: TC rs7219451: CC rs7211017: GG
|
Treatment protocol |
n/a
|
Growth protocol |
Previously transformed cryo-preserved EBV cell lines were grown as 500 ml roller cultures. Once log phase had been obtained, cells were pelleted, media discarded and a mixture of RLT buffer and β-mercaptoethanol added. Pellets were vortexed to ensure thorough re-suspension after which they were frozen at -70oC and kept at -80oC.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted in using the RNeasy Maxi Kit.
|
Label |
biotin
|
Label protocol |
Procedure used standard Affymetrix labelling protocol.
|
|
|
Hybridization protocol |
Procedure used standard Affymetrix labelling protocol.
|
Scan protocol |
Procedure used standard Affymetrix labelling protocol.
|
Description |
no additional
|
Data processing |
RMA Express
|
|
|
Submission date |
Jun 08, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Goncalo Abecasis |
E-mail(s) |
goncalo@umich.edu
|
Organization name |
University of Michigan
|
Street address |
1420 Washington Heights
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE8052 |
Genetic variants regulating ORMDL3 expression are determinants of susceptibility to childhood asthma |
|
Relations |
Reanalyzed by |
GSE64985 |
Reanalyzed by |
GSE119087 |