|
| Status |
Public on Jul 01, 2007 |
| Title |
Lymphoblastoid Cell Line for Individual 8112.4 log phase |
| Sample type |
RNA |
| |
|
| Source name |
Lymphoblastoid_cell_line
|
| Organism |
Homo sapiens |
| Characteristics |
ped: 8112
id: 4
fatid: 1
motid: 2
sex: 2
country: UK
DDAST: CONTROL
rs3744350: CC
rs16492: TT
rs16235: AG
rs16238: CA
rs558317: CC
rs498973: CC
rs16495: CC
rs12946701: AA
rs16519: GG
rs16522: TT
rs544198: TG
rs657672: CC
rs657723: TC
rs16527: TC
rs3025203: GG
rs801259: TG
rs3744353: GG
rs2301775: AG
rs16531: TT
rs521633: AC
rs16530: CC
rs486512: CC
rs1106769: AA
rs2061342: GT
rs7214151: TC
rs7208487: TG
rs9906612: AC
rs4795369: AG
rs11655550: TT
rs4611492: TT
rs12450559: AG
rs12453682: TC
rs1877032: AA
rs879606: GA
rs9892427: TT
rs1877031: TC
rs2271308: GA
rs931992: AC
rs2941503: GA
rs2941504: GA
rs1565922: CT
rs907092: GA
rs9911688: CC
rs9303277: CT
rs2060941: GT
rs9908694: CC
rs11557467: GT
rs8067378: AG
rs2305480: CT
rs2290400: AG
rs7216389: TC
rs3744246: CT
rs4795405: CT
rs8079416: TT
rs4795408: GG
rs1007654: GA
rs3902025: AC
rs3894194: CC
rs3859192: CC
rs921651: GG
rs8066582: CC
rs8080546: AA
rs2071369: CC
rs2302776: AA
rs3934886: CC
rs11078936: TT
rs868150: CC
rs7502966: TC
rs1568400: GG
rs939348: CC
rs3744805: GG
rs2071427: GG
rs2269457: AA
rs2071570: GG
rs7211770: AA
rs2280400: CT
rs13706: GA
rs2715553: TT
rs4890109: GG
rs2715556: GG
rs2715555: CC
rs13695: GA
rs11650680: CC
rs2015561: TT
rs538399: TT
rs7214466: CC
rs8078319: CC
rs749906: CT
rs4890115: CC
rs2290207: AG
rs7214311: AG
rs896136: TT
rs9675320: TT
rs2023906: AA
rs3136685: GG
rs608517: AG
rs1358175: TC
rs757411: TC
rs11659024: AG
rs1474454: CT
rs1526603: CC
rs2014704: CA
rs2462961: TC
rs2429553: AA
rs1880832: AG
rs9915334: TC
rs9303295: AA
rs1405334: CC
rs4890126: AG
rs4890127: CT
rs2109217: CA
rs8079180: GG
rs2429542: AA
rs975078: AG
rs9898164: AA
rs719867: CT
rs981684: AG
rs12453124: CC
rs4132126: AA
rs7209228: CC
rs7219451: TC
rs7211017: AG
|
| Treatment protocol |
n/a
|
| Growth protocol |
Previously transformed cryo-preserved EBV cell lines were grown as 500 ml roller cultures. Once log phase had been obtained, cells were pelleted, media discarded and a mixture of RLT buffer and β-mercaptoethanol added. Pellets were vortexed to ensure thorough re-suspension after which they were frozen at -70oC and kept at -80oC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted in using the RNeasy Maxi Kit.
|
| Label |
biotin
|
| Label protocol |
Procedure used standard Affymetrix labelling protocol.
|
| |
|
| Hybridization protocol |
Procedure used standard Affymetrix labelling protocol.
|
| Scan protocol |
Procedure used standard Affymetrix labelling protocol.
|
| Description |
no additional
|
| Data processing |
RMA Express
|
| |
|
| Submission date |
Jun 08, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Goncalo Abecasis |
| E-mail(s) |
goncalo@umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1420 Washington Heights
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8052 |
Genetic variants regulating ORMDL3 expression are determinants of susceptibility to childhood asthma |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
