|
| Status |
Public on Jul 01, 2007 |
| Title |
Lymphoblastoid Cell Line for Individual 8051.3 log phase |
| Sample type |
RNA |
| |
|
| Source name |
Lymphoblastoid_cell_line
|
| Organism |
Homo sapiens |
| Characteristics |
ped: 8051
id: 3
fatid: 1
motid: 2
sex: 1
country: UK
DDAST: CASE
rs3744350: CC
rs16492: TT
rs16235: AG
rs16238: CA
rs558317: CC
rs498973: CC
rs16495: CC
rs12946701: AA
rs16519: GG
rs16522: TT
rs544198: TT
rs657672: CC
rs657723: TT
rs16527: TT
rs3025203: GG
rs801259: TT
rs3744353: GG
rs2301775: AA
rs16531: TT
rs521633: AC
rs16530: CC
rs486512: CC
rs1106769: AA
rs2061342: GG
rs7214151: TC
rs7208487: TT
rs9906612: AA
rs4795369: AA
rs11655550: TT
rs4611492: TT
rs12450559: AA
rs12453682: TT
rs1877032: AA
rs879606: GG
rs9892427: TT
rs1877031: TT
rs2271308: GG
rs931992: AA
rs2941503: GG
rs2941504: GG
rs1565922: CC
rs907092: GG
rs9911688: CC
rs9303277: CC
rs2060941: GG
rs9908694: CC
rs11557467: GG
rs8067378: AA
rs2305480: CC
rs2290400: AA
rs7216389: TT
rs3744246: CC
rs4795405: CC
rs8079416: CC
rs4795408: AA
rs1007654: GG
rs3902025: AA
rs3894194: TT
rs3859192: TC
rs921651: AA
rs8066582: TC
rs8080546: CC
rs2071369: CC
rs2302776: AG
rs3934886: TT
rs11078936: TC
rs868150: TC
rs7502966: TT
rs1568400: AA
rs939348: TC
rs3744805: GG
rs2071427: GG
rs2269457: AA
rs2071570: GT
rs7211770: AG
rs2280400: CC
rs13706: GG
rs2715553: CT
rs4890109: GG
rs2715556: GG
rs2715555: CT
rs13695: GA
rs11650680: CT
rs2015561: TC
rs538399: TC
rs7214466: TC
rs8078319: CT
rs749906: CT
rs4890115: CC
rs2290207: GG
rs7214311: AA
rs896136: TT
rs9675320: TT
rs2023906: AG
rs3136685: GG
rs608517: AG
rs1358175: TT
rs757411: TT
rs11659024: AA
rs1474454: CC
rs1526603: CC
rs2014704: CC
rs2462961: TT
rs2429553: GG
rs1880832: AG
rs9915334: TT
rs9303295: AG
rs1405334: CT
rs4890126: GG
rs4890127: CT
rs2109217: CA
rs8079180: GG
rs2429542: AA
rs975078: AG
rs9898164: AA
rs719867: CT
rs981684: AG
rs12453124: CC
rs4132126: AA
rs7209228: CC
rs7219451: TC
rs7211017: AG
|
| Treatment protocol |
n/a
|
| Growth protocol |
Previously transformed cryo-preserved EBV cell lines were grown as 500 ml roller cultures. Once log phase had been obtained, cells were pelleted, media discarded and a mixture of RLT buffer and β-mercaptoethanol added. Pellets were vortexed to ensure thorough re-suspension after which they were frozen at -70oC and kept at -80oC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted in using the RNeasy Maxi Kit.
|
| Label |
biotin
|
| Label protocol |
Procedure used standard Affymetrix labelling protocol.
|
| |
|
| Hybridization protocol |
Procedure used standard Affymetrix labelling protocol.
|
| Scan protocol |
Procedure used standard Affymetrix labelling protocol.
|
| Description |
no additional
|
| Data processing |
RMA Express
|
| |
|
| Submission date |
Jun 08, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Goncalo Abecasis |
| E-mail(s) |
goncalo@umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1420 Washington Heights
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8052 |
Genetic variants regulating ORMDL3 expression are determinants of susceptibility to childhood asthma |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
