|
Status |
Public on Jul 01, 2007 |
Title |
Lymphoblastoid Cell Line for Individual 8038.3 log phase |
Sample type |
RNA |
|
|
Source name |
Lymphoblastoid_cell_line
|
Organism |
Homo sapiens |
Characteristics |
ped: 8038 id: 3 fatid: 1 motid: 2 sex: 1 country: UK DDAST: CASE rs3744350: CC rs16492: TT rs16235: GG rs16238: AA rs558317: CC rs498973: CC rs16495: CC rs12946701: AA rs16519: GG rs16522: TC rs544198: TG rs657672: CT rs657723: TC rs16527: TC rs3025203: GG rs801259: TG rs3744353: GG rs2301775: AA rs16531: TC rs521633: AA rs16530: CC rs486512: CT rs1106769: AA rs2061342: TT rs7214151: TT rs7208487: GG rs9906612: CC rs4795369: GG rs11655550: TT rs4611492: TT rs12450559: GG rs12453682: CC rs1877032: AA rs879606: GA rs9892427: TT rs1877031: TC rs2271308: GA rs931992: AC rs2941503: GA rs2941504: GA rs1565922: CT rs907092: GA rs9911688: CC rs9303277: CT rs2060941: GG rs9908694: CC rs11557467: GT rs8067378: AG rs2305480: CT rs2290400: AG rs7216389: TC rs3744246: CC rs4795405: CC rs8079416: CT rs4795408: AG rs1007654: GG rs3902025: AA rs3894194: TC rs3859192: TC rs921651: AG rs8066582: TC rs8080546: CA rs2071369: CC rs2302776: AG rs3934886: TC rs11078936: TT rs868150: TC rs7502966: TC rs1568400: AG rs939348: CC rs3744805: GG rs2071427: GG rs2269457: AA rs2071570: GG rs7211770: AA rs2280400: CC rs13706: GG rs2715553: CT rs4890109: GG rs2715556: GG rs2715555: CC rs13695: GG rs11650680: CC rs2015561: TT rs538399: TC rs7214466: TC rs8078319: CC rs749906: CC rs4890115: CC rs2290207: GG rs7214311: AA rs896136: TT rs9675320: TT rs2023906: AA rs3136685: GG rs608517: AG rs1358175: TC rs757411: TC rs11659024: AG rs1474454: CT rs1526603: CC rs2014704: CA rs2462961: TC rs2429553: AA rs1880832: GG rs9915334: CC rs9303295: AA rs1405334: CC rs4890126: AA rs4890127: CC rs2109217: CC rs8079180: GG rs2429542: AA rs975078: AA rs9898164: AA rs719867: CC rs981684: AA rs12453124: CC rs4132126: AG rs7209228: TC rs7219451: TC rs7211017: GG
|
Treatment protocol |
n/a
|
Growth protocol |
Previously transformed cryo-preserved EBV cell lines were grown as 500 ml roller cultures. Once log phase had been obtained, cells were pelleted, media discarded and a mixture of RLT buffer and β-mercaptoethanol added. Pellets were vortexed to ensure thorough re-suspension after which they were frozen at -70oC and kept at -80oC.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted in using the RNeasy Maxi Kit.
|
Label |
biotin
|
Label protocol |
Procedure used standard Affymetrix labelling protocol.
|
|
|
Hybridization protocol |
Procedure used standard Affymetrix labelling protocol.
|
Scan protocol |
Procedure used standard Affymetrix labelling protocol.
|
Description |
no additional
|
Data processing |
RMA Express
|
|
|
Submission date |
Jun 08, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Goncalo Abecasis |
E-mail(s) |
goncalo@umich.edu
|
Organization name |
University of Michigan
|
Street address |
1420 Washington Heights
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE8052 |
Genetic variants regulating ORMDL3 expression are determinants of susceptibility to childhood asthma |
|
Relations |
Reanalyzed by |
GSE64985 |
Reanalyzed by |
GSE119087 |