|
| Status |
Public on Feb 25, 2009 |
| Title |
Primary hepatocyte cadmium, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
primary hepatocyte in culture from rat 3
|
| Organism |
Rattus norvegicus |
| Characteristics |
agent: cadmium
dose: 4μM
time: 3hrs
|
| Treatment protocol |
Primary rat hepatocytes in culture are incubated with low concentration of cadmium chloride (4μM) for 3 hours or nothing (culture media only).
|
| Growth protocol |
Primary rat heaptocytes were isolated, plated in culture dishes and incubated overnight prior to treatment of either control group (culture media) or cadmium group.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 μg total RNA (Expression Analysis Technical Manual, 701022 Rev. 2, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 μg of cRNA were hybridized for 16 hrs at 45°C on RAE 230A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
gene expression data from primary rat hepatocyte in culture
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 04, 2007 |
| Last update date |
Mar 29, 2010 |
| Contact name |
Susan R Stapleton |
| E-mail(s) |
susan.stapleton@wmich.edu
|
| Phone |
269-387-2853
|
| Fax |
269-387-2909
|
| Organization name |
Western Michigan University
|
| Department |
Chemistry
|
| Street address |
3425 Wood Hall
|
| City |
Kalamazoo |
| State/province |
MI |
| ZIP/Postal code |
49008 |
| Country |
USA |
| |
|
| Platform ID |
GPL341 |
| Series (1) |
| GSE8013 |
Gene expression profiling under low dose and short incubation time of cadmium in primary rat hepatocyte in culture |
|
