|
| Status |
Public on Aug 31, 2016 |
| Title |
Microdissected E11.5 lung epithelial tip biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Tip epithelial progenitor cells from E11.5 developing lung
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E11.5
cell type: Lung epithelial tip progenitor cells
cell isolation: Cells microdissected from embryonic lung
embryo genotype: Id2-CreER; Rosa26R-fGFP
|
| Treatment protocol |
Lungs were dissected from E11.5 embryos and tip epithelium was microdissected following a 5 minute treatment with 16U/ml dispase. For E17.5 cells, pregnant females were injected with 150 micrograms per gram body weight at E16.5. E17.5 embryos were dissected and GFP+ lungs selected under GFP-dissecting microscope. Lungs were dissociated to single cells using trypsin/EDTA and GFP+ cells isolated by flow cytometry.
|
| Growth protocol |
Timed matings of Id2-CreER/+heterozygotes with Rosa26R-fGFP/fGFP homozygotes. Noon on day of plug = E0.5.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA using Qaigen RNEasy Mini Kit as per manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol using Ovation RNA Amplification Kit V2 and FL-Ovation Biotin V2 (NuGEN)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Data processing |
Bioconductor
|
| |
|
| Submission date |
Dec 09, 2015 |
| Last update date |
Aug 31, 2016 |
| Contact name |
Emma Louise Rawlins |
| E-mail(s) |
e.rawlins@gurdon.cam.ac.uk
|
| Organization name |
University Of Cambridge
|
| Department |
Gurdon Institute
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE75860 |
Expression data from mouse embryonic lung epithelial tip progenitor cells |
|
