|
| Status |
Public on May 01, 2016 |
| Title |
BURN_IP |
| Sample type |
SRA |
| |
|
| Source name |
Burn injury_DRG neurons
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: wild type
gender: male
age: 6-8 weeks
sample group: three days after the burn injury
tissue: L3, L4, L5 whole DRG neurons
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole DRGs were removed and immediately put into RNALater and cut into pieces in RNA later. RNA was extracted using the Rneasy Mini kit (Qiagen)
RNA libraries were prepared using TruSeq stranded total RNA library preparation.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Alignment: STAR aligner (version star/2.3.0e) and samtools
Mapping: Mapped to Ensembl Mus musculus mm10 genome (version GRCm38)
Counts: Generated using HTSeq_count in the HTSeq package
Genome_build: mm10
Supplementary_files_format_and_content: .txt files, tab-delimited.
|
| |
|
| Submission date |
Dec 03, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Kathleen Yin |
| E-mail(s) |
kathleen.yin@uq.net.au
|
| Organization name |
University of Queensland
|
| Department |
Institute of Molecular Biosciences
|
| Street address |
306 Carmody Rd
|
| City |
St Lucia |
| State/province |
Queensland |
| ZIP/Postal code |
4067 |
| Country |
Australia |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE75691 |
Transcriptomic and behavioural characterisation of a mouse model of burns pain |
|
| Relations |
| BioSample |
SAMN04316863 |
| SRA |
SRX1462881 |
