|
| Status |
Public on Nov 03, 2015 |
| Title |
GES-1 cells_hp26695_24h_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Human gastric epithelial cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human gastric epithelial cell, GES-1 cell line
|
| Treatment protocol |
GES-1 cells were seeded in six-well cell culture plates before treated. The following day, 80% confluent monolayer cells were washed in PBS, and the medium was replaced with fresh medium. Then, H.pylori was added to cells for 24 hours at a multiplicity of infection (MOI) of 100 as experimental groups. Cells without H.pylori infection were maintained for indicated time periods as control groups. After 24hr-infection, cells were washed in PBS to wash away the non-adherent H.pylori, then harvested in Trizol reagent and preserved at -80°C until RNA isolation.
|
| Growth protocol |
GES-1 cells were maintained in humidified air with 5% CO2 at 37°C before use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated samples were prepared according to the user manual of GeneChip® WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
After fragmentation, samples were hybridized in GeneChip® Hybridization Oven 645 according to the user manual of GeneChip® Hybridization, Wash and Stain Kit.
|
| Scan protocol |
After the wash protocols completing, the probe arrays were scanned by using the GeneChip® Scanner 3000 7G according to instructions for scanning probe arrays.
|
| Description |
Coding and non-coding gene expression data of GES-1 cells from H.pylori-infected group 1.
|
| Data processing |
The data were analyzed with Affymetrix Expression Console™ Software (version 1.3).
|
| |
|
| Submission date |
Nov 02, 2015 |
| Last update date |
Nov 03, 2015 |
| Contact name |
Hong ZHU |
| E-mail(s) |
emeraldzh@sina.com
|
| Phone |
8615951231250
|
| Organization name |
Jiangsu University
|
| Street address |
No.301,Xue Fu Road, Jing Kou District
|
| City |
Zhen Jiang |
| State/province |
Jiang Su |
| ZIP/Postal code |
212013 |
| Country |
China |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE74577 |
LncRNA and mRNA expression profiles from human GES-1 cells in response to Helicobacter pylori infection |
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