|
| Status |
Public on May 04, 2016 |
| Title |
S13_Pre_hypoxic bedrest (HBR) |
| Sample type |
RNA |
| |
|
| Source name |
Human skeletal muscle, Vastus lateralis
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Vastus lateralis
gender: Male
condition: hypoxic bedrest (HBR)
subject: 13
intervention_number: Second
time: Pre
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Biopsies were obtained from the vastus lateralis muscle using the Bergström needle technique. The biopsies were immediately stored in liquid nitrogen until processing. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix miRNA_4.1 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Not provided
|
| Description |
Subject 13, Second Intervention: hypoxic bedrest (HBR), Pre
|
| Data processing |
The probe-set level intensities of the arrays were normalized using the Robust Multi-array Analysis method (RMA) implemented withinAffymetrix Expression Console version 1.4.1.
|
| |
|
| Submission date |
Oct 29, 2015 |
| Last update date |
May 04, 2016 |
| Contact name |
Eric Rullman |
| E-mail(s) |
eric.rullman@gmail.com
|
| Phone |
+46858586777
|
| Organization name |
Karolinska Institutet
|
| Department |
Laboratory Medicine
|
| Lab |
Clinical Physiology
|
| Street address |
C1:82 Karolinska University Hospital, Huddinge
|
| City |
Stockholm |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE74469 |
Effects of bedrest and hypoxia on human skeletal muscle miRNA expression |
|
