|
| Status |
Public on Nov 25, 2015 |
| Title |
CMP_Flt3 |
| Sample type |
SRA |
| |
|
| Source name |
CMP Flt3+ Csf1r+
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 6-8 wks
tissue: bone marrow
cell type: common myeloid progenitor (CMP)
cell subtype: CMP Flt3+ Csf1r+
chip antibody: K4me2 (Abcam-ab7766)
|
| Treatment protocol |
no treatment
|
| Growth protocol |
6 to 8 weeks-old mice housed at the Weizmann Institute animal facility
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.
Lara-Astiaso D, Weiner A, Lorenzo-Vivas E, Zaretsky I et al. Immunogenetics. Chromatin state dynamics during blood formation. Science 2014 Aug 22;345(6199):943-9. PMID: 25103404
indexing-first chromatin immunoprecipitation
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
bcl2fastq/2.15.0.4
alignment using bowtiebowtie2/2.1.0 using default parameters
Genome_build: mm9
Supplementary_files_format_and_content: bigWig
|
| |
|
| Submission date |
Sep 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Ido Amit |
| E-mail(s) |
ido.amit@weizmann.ac.il
|
| Phone |
972-8-9343338
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Immunology
|
| Street address |
234 Herzl st.
|
| City |
Rehovot |
| ZIP/Postal code |
760001 |
| Country |
Israel |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE72858 |
Transcriptional heterogeneity and lineage commitment in myeloid progenitors [ChIP-seq] |
| GSE72859 |
Transcriptional heterogeneity and lineage commitment in myeloid progenitors |
|
| Relations |
| BioSample |
SAMN04044124 |
| SRA |
SRX1213161 |
