|
| Status |
Public on Aug 29, 2016 |
| Title |
Unknown_45 [validation] |
| Sample type |
RNA |
| |
|
| Source name |
Whole Blood from patient with infection of uncertain bacterial or viral aetiology
|
| Organism |
Homo sapiens |
| Characteristics |
category: Uncertain
dataset: Validation
gender: Female
tissue: Whole Blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard PAXgene® blood RNA extraction kit protocol (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Standard Illumina TotalPrep RNA Amplification kits (Applied Biosystems).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridisation protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Data processing |
The data were normalised using RSN, after background subtraction and filtering for transcripts common to Illumina HT12-V4
The software used for data normalization was 'R' Language and Environment for Statistical Computing (R) 3.1.2
|
| |
|
| Submission date |
Sep 08, 2015 |
| Last update date |
Aug 29, 2016 |
| Contact name |
Victoria Wright |
| E-mail(s) |
v.wright@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Paediatrics
|
| Lab |
Wright Fleming Institute
|
| Street address |
Norfolk Place
|
| City |
London |
| ZIP/Postal code |
W2 1PG |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE72810 |
Diagnosis of childhood bacterial and viral infection using host RNA expression [validation set] |
| GSE72829 |
Diagnosis of childhood bacterial and viral infection using host RNA expression |
|
