|
| Status |
Public on Sep 30, 2015 |
| Title |
2_2480UCon_IL2_STAT5_i81 |
| Sample type |
SRA |
| |
|
| Source name |
CD8 T cell
|
| Organism |
Mus musculus |
| Characteristics |
cell type: effector CD8 T cell
strain: C57BL/6
treatment: 0.5 ug/ml IL-2 for 1 hour
antibody: anti-STAT5/R & D Systems, #PA-ST5A, lot #RU07
|
| Treatment protocol |
Adoptively transferred OT-I CD8 T cells were primed in vivo with SIINFEKL peptide and CD134 plus CD137 costimulatory agonists and FACS sorted from spleens 4 days later and then treated in vitro with or without 0.5 ug/ml IL-2 for 1 hour
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Treated cells were snap frozen and shipped to ActiveMotif, Inc. who performed the STAT5A ChIP-seq analysis according to their FactorPath™ assay.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Data processing |
The 50-nt sequence reads identified by the Sequencing Service (using
Illumina’s Hi-Seq) are mapped to the genome using the BWA algorithm with default settings.
Alignment information for each read is stored in the BAM format. Only reads that pass Illumina’s purity filter, align with no more than 2 mismatches, and map uniquely to the genome are used in the subsequent analysis. In addition, unless stated otherwise, duplicate reads (“PCR duplicates”) are
Genome_build: removed. Approximately 17 million non-duplicate reads per sample were alligned to the mouse genome (mm10).
|
| |
|
| Submission date |
Aug 31, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Anthony T Vella |
| E-mail(s) |
vella@uchc.edu
|
| Organization name |
University of Connecticut Health Center
|
| Department |
Immunology
|
| Lab |
Anthony T Vella
|
| Street address |
263 Farmington Avenue
|
| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06030 |
| Country |
USA |
| |
|
| Platform ID |
GPL9185 |
| Series (1) |
| GSE72565 |
Binding of STAT5 upon IL-2 treatment to genomic sites in mouse CD8 T cells costimulated in vivo through CD134 plus CD137 |
|
| Relations |
| Reanalyzed by |
GSE111902 |
| BioSample |
SAMN04023005 |
| SRA |
SRX1176004 |
