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Sample GSM1862825 Query DataSets for GSM1862825
Status Public on Nov 13, 2016
Title WT_Epidermis_Neonate_replicate3
Sample type RNA
Source name WT, Epidermis, Neonate, replicate3
Organism Mus musculus
Characteristics genotype/variation: wild type
tissue: epidermis
strain: mix
age: postnatal day 0
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from epidermis using RNeasy Micro kit (Qiagen).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using the Quick Amp Labeling kit (Agilent) according to the manufacturer's protocol.
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Description Gene expression in wild-type epidermis at postnatal day 0, replicate3
Data processing The data were normalized by percentile shift, using GeneSpring GX software (Silicon Genetics).
Submission date Aug 27, 2015
Last update date Nov 13, 2016
Contact name Eri H Kobayashi
Organization name Tohoku University Graduate School of Medicine
Department Medical Biochemistry
Street address 2-1 Seiryo-machi, Aobaku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL7202
Series (1)
GSE72449 AhR links air pollution and atopic dermatitis via Artemin induction

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.53696346
DarkCorner 0.1742487
A_52_P616356 0.15930462
A_52_P580582 0.48939943
A_52_P403405 0.0073452
A_52_P819156 -0.7738004
A_51_P331831 -0.35792994
A_51_P430630 0.17714596
A_52_P502357 0.1796155
A_52_P299964 -0.226933
A_51_P356389 0.3294623
A_52_P684402 -0.017115116
A_51_P414208 0.072416306
A_51_P280918 0.11330986
A_52_P613688 0.6951022
A_52_P258194 0.18537235
A_52_P229271 -0.3168521
A_52_P214630 0.32105732
A_52_P579519 0.16337466
A_52_P979997 0.19727421

Total number of rows: 41267

Table truncated, full table size 975 Kbytes.

Supplementary file Size Download File type/resource
GSM1862825_WT_Epidermis_Neonate_replicate3.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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