|
| Status |
Public on Jun 06, 2008 |
| Title |
MSC #5, technical rep-amplification- rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mesenchymal stem cells, 5th passage
|
| Organism |
Homo sapiens |
| Characteristics |
two-cycle
bone marrow mesenchymal stem cells
|
| Treatment protocol |
Cells were lysed in 600 ul of buffer RLT (QIAGEN).
|
| Growth protocol |
Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 5
|
| Data processing |
The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
|
| |
|
| Submission date |
Apr 26, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Shihori Tanabe |
| Organization name |
National Institute of Health Sciences
|
| Department |
Division of Risk Assessment
|
| Street address |
3-25-26, Tonomachi, Kawasaki-ku
|
| City |
Kawasaki |
| State/province |
Kanagawa |
| ZIP/Postal code |
210-9501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7637 |
Expression data from human mesenchymal stem cells (#4F1560) |
|
| Relations |
| Reanalyzed by |
GSE119087 |
