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Status
Public on Jan 30, 2017
Title
CD14+ monocytes from peripheral blood (healthy control HC-3)
Sample type
RNA
Source name
CD14+ monocytes from peripheral blood
Organism
Homo sapiens
Characteristics
disease state: control individual: HC-3 tissue: peripheral blood cell type: CD14+ monocyte
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated using the standard TRIzol protocol (Invitrogen, Carlsbad, CA), but with subsequent RNA clean-up of the water phase using the RNeasy Qiagen MinElute kit (Qiagen, Germantown, MD). The quality of the RNA was confirmed on the Agilent Bioanalyzer using RNA Nano chips (Agilent Technologies, Santa Clara, CA).
Label
biotin
Label protocol
Affymetrix in vitro transcription
Hybridization protocol
Affymetrix EukGE-WS2v5
Scan protocol
Affymetrix cel analysis
Description
1636_(HG-U133_Plus_2)
Data processing
Bioconductor/R RMA
Submission date
Jul 27, 2015
Last update date
Jan 30, 2017
Contact name
Klaus Stensgaard Frederiksen
Organization name
Novo Nordisk A/S
Department
Molecular Genetics
Street address
Novo Nordisk Park F9.1.07
City
Måløv
ZIP/Postal code
DK-2760
Country
Denmark
Platform ID
GPL16268
Series (1)
GSE71370
Profiling of CD14+ monocytes from paired rheumatoid arthritis (RA)-patient peripheral blood and synovial fluid samples
Data table header descriptions
ID_REF
VALUE
Log2 RMA signal
Data table
ID_REF
VALUE
AFFX-BioB-3_at
4.19848
AFFX-BioB-5_at
4.48083
AFFX-BioB-M_at
5.00656
AFFX-BioC-3_at
8.93386
AFFX-BioC-5_at
8.69102
AFFX-BioDn-3_at
11.225
AFFX-BioDn-5_at
10.5069
AFFX-CreX-3_at
12.9971
AFFX-CreX-5_at
12.7
AFFX-DapX-3_at
10.238
AFFX-DapX-5_at
7.23181
AFFX-DapX-M_at
9.2448
AFFX-HSAC07/X00351_3_at
12.5541
AFFX-HSAC07/X00351_5_at
9.8709
AFFX-HSAC07/X00351_M_at
11.7735
AFFX-HUMGAPDH/M33197_3_at
11.8913
AFFX-HUMGAPDH/M33197_5_at
11.4775
AFFX-HUMGAPDH/M33197_M_at
11.6904
AFFX-HUMISGF3A/M97935_3_at
9.5238
AFFX-HUMISGF3A/M97935_5_at
5.30789
Total number of rows: 19598 Table truncated, full table size 514 Kbytes .Supplementary file
Size
Download
File type/resource
GSM1833160_1636_HG-U133_Plus_2_.CEL.gz
4.1 Mb
(ftp) (http)
CEL
Processed data included within Sample table