|
Status |
Public on Jul 23, 2015 |
Title |
WB_non-statin_pt_244 |
Sample type |
RNA |
|
|
Source name |
PAXgene whole blood mRNA
|
Organism |
Homo sapiens |
Characteristics |
statin user (y/n): N disease: COPD age: 51 Sex: M smoking status: Former smoker fev1% predicted: 52.3 fev1/fvc: 51.86
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen PAXgene Blood miRNA Kit (Cat# 763134), automated processing using the Qiagen QIAcube robotic workstation (Cat# 9001292). Protocol to amplify RNA-Ambion WT Expression RNA Amplification Kit (Part# 4440536)
|
Label |
biotin
|
Label protocol |
Affymetrix GeneChip HT WT Terminal Labeling Kit (Part# 901648)
|
|
|
Hybridization protocol |
Affymetrix GeneTitan Hybridization, Wash and Stain Kit for WT Array Plates (Part# 901622)
|
Scan protocol |
Affymetrix GeneChip Command Console (AGCC) version 4.1.2.1567G for scanning, image acquisition and image processing on the Affymetrix GeneTitan Multi-Channel(MC) Instrument.
|
Data processing |
RMA normalization with Farms bioconductor package to filter out non-informative probe sets
|
|
|
Submission date |
Jul 22, 2015 |
Last update date |
Aug 16, 2016 |
Contact name |
Bruce Maxwell McManus |
E-mail(s) |
Bruce.McManus@hli.ubc.ca
|
Phone |
604-806-8586
|
Organization name |
PROOF Centre of Excellence
|
Department |
UBC James Hogg Research Centre
|
Street address |
1081 Burrard Street
|
City |
Vancouver |
State/province |
BC |
ZIP/Postal code |
V6Z 1Y6 |
Country |
Canada |
|
|
Platform ID |
GPL11532 |
Series (1) |
GSE71220 |
The effect of statins on blood gene expression in COPD |
|