|
| Status |
Public on Jan 03, 2008 |
| Title |
CP35BA_subject 35 |
| Sample type |
RNA |
| |
|
| Source name |
healthy human subject
|
| Organism |
Homo sapiens |
| Characteristics |
dose=0
time=0hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. Residual phenol were removed using Qiagen's spin columns.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix two-cycle protocol from 50ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133plus2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 scanner
|
| Description |
Gene expression data from subject at time 0h exposed to filtered air
|
| Data processing |
The data were analyzed with BioConductor GCRMA using the gcrma package.
|
| |
|
| Submission date |
Apr 05, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
James William MacDonald |
| E-mail(s) |
jmacdon@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7462 |
Gene expression of diesel exhaust inhalation in peripheral blood mononuclear cells from healthy human volunteers |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
