|
| Status |
Public on Mar 08, 2010 |
| Title |
Mouse_Parthenogenetic_Diploid_Blastocysts |
| Sample type |
RNA |
| |
|
| Source name |
Parthenogenetic_Diploid_Blastocysts_96hours
|
| Organism |
Mus musculus |
| Characteristics |
mouse embryos 96 hours in culture, maternal genome only
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion RNAqueous MICRO kit
|
| Label |
Biotin
|
| Label protocol |
Affymetrix two-cycle amplification protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
GeneChip scanner 3000, 1.5 micrometer resolution
|
| Description |
Comparison of mouse development in normal fertilized embryos and parthenogenetic embryos. This experiment looked at day 4 blastocysts which are indistinguishable morphologically but differ in their gene expression profiles.
|
| Data processing |
Signal values calculated in MAS 5.0 and scaled to generate a trimmed mean intensity of 500. The data provided here has not been processed further.
|
| |
|
| Submission date |
Mar 29, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Sean Yoder |
| E-mail(s) |
sean.yoder@moffitt.org
|
| Phone |
813-745-1917
|
| Organization name |
Moffitt Cancer Center & Research Institute
|
| Department |
Molecular Genomics Core Facility
|
| Lab |
SRB-2
|
| Street address |
12902 Magnolia Dr.
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33612 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7399 |
Gene Expression Profiling of Blastocysts Reveals Early Differences Associated with Parthenogenetic Embryos |
|
| Relations |
| Reanalyzed by |
GSE119085 |
