|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Dec 04, 2007 |
Title |
ActCD8TGF, rep1 |
Sample type |
RNA |
|
|
Source name |
CD8 T cells activated in vitro with TGFbeta
|
Organism |
Mus musculus |
Characteristics |
Genetic background: BDC2.5/NOD Age: 4-6 wks Number of mice per chip: 3-6 Tissue: Spleen and LN (cervical, mesenteric) Sort: B220-, CD11b-, CD4-, CD69-, CD25-, CD62Lhi, CD8a+ Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25 ng/ml) for 4 days Post-culture sort: CD8a hi expressers
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
|
Label |
Biotin
|
Label protocol |
BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
|
|
|
Hybridization protocol |
Per Joslin Diabetes Center microarray core facility.
|
Scan protocol |
Per Joslin Diabetes Center microarray core facility.
|
Description |
jos3284.CEL
|
Data processing |
RMA
|
|
|
Submission date |
Mar 22, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
CBDM Lab |
E-mail(s) |
cbdm@hms.harvard.edu
|
Phone |
617-432-7747
|
Organization name |
Harvard Medical School
|
Department |
Microbiology and Immunobiology
|
Lab |
CBDM
|
Street address |
77 Avenue Louis Pasteur
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02215 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE7460 |
Contribution of Foxp3 to the Treg signature |
|
Relations |
Reanalyzed by |
GSE119085 |
Data table header descriptions |
ID_REF |
|
VALUE |
RMA-unlogged |
Data table |
ID_REF |
VALUE |
1415670_at |
181.3917674 |
1415671_at |
681.5385941 |
1415672_at |
3907.832636 |
1415673_at |
284.1273414 |
1415674_a_at |
299.1036401 |
1415675_at |
401.2583153 |
1415676_a_at |
698.3028664 |
1415677_at |
374.9623636 |
1415678_at |
847.1988379 |
1415679_at |
2529.215354 |
1415680_at |
272.2025462 |
1415681_at |
257.2045031 |
1415682_at |
150.0314379 |
1415683_at |
935.5818223 |
1415684_at |
378.6058289 |
1415685_at |
150.4531869 |
1415686_at |
1295.439267 |
1415687_a_at |
1929.592045 |
1415688_at |
1584.27135 |
1415689_s_at |
675.0712057 |
Total number of rows: 45101
Table truncated, full table size 1026 Kbytes.
Supplementary file |
Size |
Download |
File type/resource |
GSM176881.CEL.gz |
3.1 Mb |
(ftp)(http) |
CEL |
Processed data included within Sample table |
|
|
|
|
|