|
| Status |
Public on Dec 31, 2007 |
| Title |
Tonsil epithelium 2-batch 2 |
| Sample type |
RNA |
| |
|
| Source name |
Hyperplastic Tonsil of Sleap Apnea Adult Patient
|
| Organism |
Homo sapiens |
| Characteristics |
Tonsil Epithelium
|
| Treatment protocol |
Tissues were snap frozen emediately after surgery
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Laser Capture Microdisection (Leica, LMD) of the epithelium and RNA extraction using the Qiagen RNAeasy Micro-Kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 30ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
|
| Description |
Laser Capture Microdissected Epithelium of Tonsil from healthy donors.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 08, 2007 |
| Last update date |
Jun 22, 2021 |
| Contact name |
Niki Moutsopoulos |
| E-mail(s) |
nmoutsopoulos@dir.nidr.nih.gov
|
| Organization name |
NIDCR
|
| Street address |
30 convent Dr
|
| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7224 |
Gene expression in tonsil and oral epithelia |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
