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Sample GSM173114 Query DataSets for GSM173114
Status Public on Jun 01, 2007
Title d1ERbeta6h+tet(5)UHR(3)array67 T47D cells with inducible ERbeta
Sample type RNA
 
Channel 1
Source name T-47D cells with inducible ERbeta construct
Organism Homo sapiens
Characteristics T-47D breast cancer cell line
Tissue: breast cancer cell line
Growth protocol Cells were added to 150 mm plates at a confluency of 40%; after one day, the normal medium was replaced by the medium described above, supplemented with 5% dextran coated charcoal treated FBS (DCCFBS). After 24h, 10 nM ICI 182,780 was added to the cultures and incubation proceeded for an additional 48h. For expression of ERb, tetracycline was removed 12 h before initiation of treatment with 17b-estradiol. At time 0 h the medium was changed to 0.5% DCCFBS and 10 nM of 17b-estradiol or equivalent volume of DMSO for mock-treated controls were added.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol 25 ug each of sample total RNA and human universal reference RNA (Stratagene, La Jolla, CA, USA) were labeled with Cy5-conjugated dUTP and Cy3-conjugated dUTP (PerkinElmer, Boston, MA, USA), respectively, and hybridized to the arrays using protocols established by the Patrick O. Brown Laboratory (Stanford University, USA) accessible at http://cmgm.stanford.edu/pbrown/protocols/index.html.
 
Channel 2
Source name Universal human reference RNA (Stratagene)
Organism Homo sapiens
Characteristics Purchased from Stratagene
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol 25 ug each of sample total RNA and human universal reference RNA (Stratagene, La Jolla, CA, USA) were labeled with Cy5-conjugated dUTP and Cy3-conjugated dUTP (PerkinElmer, Boston, MA, USA), respectively, and hybridized to the arrays using protocols established by the Patrick O. Brown Laboratory (Stanford University, USA) accessible at http://cmgm.stanford.edu/pbrown/protocols/index.html.
 
 
Hybridization protocol Brown Lab protocol
Scan protocol Scanned on an Axon 4000B scanner.
Images were quantified using GenePix Pro Software.
Description uninduced and untreated at 6 hour following E2 treatment
Data processing Signal calculation: mean intensity minus median background and normalized by median value of all spot filtered genes.
 
Submission date Mar 06, 2007
Last update date Mar 06, 2007
Contact name Chin-Yo Lin
E-mail(s) chinyolin@byu.edu
Phone 801-422-6259
Fax 801-422-0519
Organization name Brigham Young University
Department Microbiology and Molecular Biology
Street address 753 WIDB
City Provo
State/province UT
ZIP/Postal code 84602
Country USA
 
Platform ID GPL4840
Series (1)
GSE7206 Gene expression profiling in T-47D breast cancer cells following inducible expression of estrogen receptor beta

Data table header descriptions
ID_REF
VALUE log2 ratio (Cy5/Cy3)

Data table
ID_REF VALUE
220853 0.0109
220854 0.73
220855 0.6493
220856 0.5759
220857 0.4661
220858 0.1559
220859 0.0493
220860 0.0333
220861 0.2485
220862 1.9106
220863 0.8861
220864 0.7712
220865 0.2865
220866 0.5167
220867 0.0831
220868 1.6905
220869 1.0332
220870 0.476
220871 0.6322
220872 0.9646

Total number of rows: 18858

Table truncated, full table size 263 Kbytes.




Supplementary file Size Download File type/resource
GSM173114.gpr.gz 1.8 Mb (ftp)(http) GPR

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