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Sample GSM173113 Query DataSets for GSM173113
Status Public on Jun 01, 2007
Title a4ERbeta1h-tet+E2(5)UHR(3)array99 T47D cells with inducible ERbeta
Sample type RNA
 
Channel 1
Source name T-47D cells with inducible ERbeta construct
Organism Homo sapiens
Characteristics T-47D breast cancer cell line
Tissue: breast cancer cell line
Growth protocol Cells were added to 150 mm plates at a confluency of 40%; after one day, the normal medium was replaced by the medium described above, supplemented with 5% dextran coated charcoal treated FBS (DCCFBS). After 24h, 10 nM ICI 182,780 was added to the cultures and incubation proceeded for an additional 48h. For expression of ERb, tetracycline was removed 12 h before initiation of treatment with 17b-estradiol. At time 0 h the medium was changed to 0.5% DCCFBS and 10 nM of 17b-estradiol or equivalent volume of DMSO for mock-treated controls were added.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol 25 ug each of sample total RNA and human universal reference RNA (Stratagene, La Jolla, CA, USA) were labeled with Cy5-conjugated dUTP and Cy3-conjugated dUTP (PerkinElmer, Boston, MA, USA), respectively, and hybridized to the arrays using protocols established by the Patrick O. Brown Laboratory (Stanford University, USA) accessible at http://cmgm.stanford.edu/pbrown/protocols/index.html.
 
Channel 2
Source name Universal human reference RNA (Stratagene)
Organism Homo sapiens
Characteristics Purchased from Stratagene
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol 25 ug each of sample total RNA and human universal reference RNA (Stratagene, La Jolla, CA, USA) were labeled with Cy5-conjugated dUTP and Cy3-conjugated dUTP (PerkinElmer, Boston, MA, USA), respectively, and hybridized to the arrays using protocols established by the Patrick O. Brown Laboratory (Stanford University, USA) accessible at http://cmgm.stanford.edu/pbrown/protocols/index.html.
 
 
Hybridization protocol Brown Lab protocol
Scan protocol Scanned on an Axon 4000B scanner.
Images were quantified using GenePix Pro Software.
Description induced and treated at 1 hour following E2 treatment
Data processing Signal calculation: mean intensity minus median background and normalized by median value of all spot filtered genes.
 
Submission date Mar 06, 2007
Last update date Mar 06, 2007
Contact name Chin-Yo Lin
E-mail(s) chinyolin@byu.edu
Phone 801-422-6259
Fax 801-422-0519
Organization name Brigham Young University
Department Microbiology and Molecular Biology
Street address 753 WIDB
City Provo
State/province UT
ZIP/Postal code 84602
Country USA
 
Platform ID GPL4840
Series (1)
GSE7206 Gene expression profiling in T-47D breast cancer cells following inducible expression of estrogen receptor beta

Data table header descriptions
ID_REF
VALUE log2 ratio (Cy5/Cy3)

Data table
ID_REF VALUE
220853 0.217
220854 0.1489
220855 0.5444
220856 -0.4744
220857 -0.2688
220858 0.4887
220859 -0.423
220860 -0.3709
220861 0.6053
220862 0.822
220863 -0.0635
220864 0.7772
220865 0.1976
220866
220867 0.8581
220868 0.3058
220869 -0.155
220870 -0.0019
220871 0.1349
220872 0.6782

Total number of rows: 18858

Table truncated, full table size 263 Kbytes.




Supplementary file Size Download File type/resource
GSM173113.gpr.gz 1.8 Mb (ftp)(http) GPR

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