|
| Status |
Public on Feb 24, 2010 |
| Title |
hES_SA01_1 |
| Sample type |
RNA |
| |
|
| Source name |
human embryonic stem (hES) cells line SA01, biological replicate 1
|
| Organism |
Homo sapiens |
| Characteristics |
human ES cells line from Cellartis, Sweden; commercial line derived from normal embryos
Karyotype: X,Y
|
| Growth protocol |
The hES cell lines were maintained on a feeder layer of mitomycin C-inactivated murine embryonic fibroblast (MEF) cells. The culture media (DMEM/F12 supllemented with 20% KRS and 4ng/ml bFGF) was changed daily and routine passages were performed by mechanical cutting of hES cells on a fresh feeder layer every 4-5 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the cells using the RNeasy Mini kit (Qiagen) according to the manufacturer protocol
|
| Label |
Biotin
|
| Label protocol |
100 ng of total RNA was used to generate biotinylated cRNA using the GeneChip® Two-Cycle Target Labeling and Control Reagents from Affymetrix according to the manufactures protocol
|
| |
|
| Hybridization protocol |
Hybridisation, washing and staining procedures of the Affymetrix GeneChip® Human Genome U133 Plus 2.0 were carried out as described in the Affymetrix technical manual (see manufacturer's web site)
|
| Scan protocol |
Scanning procedures of the Affymetrix GeneChip® Human Genome U133 Plus 2.0 were carried out as described in the Affymetrix technical manual (see manufacturer's web site)
|
| Description |
gene expression data from undifferentiated hES cells line SA01
|
| Data processing |
The statistical treatment of the expression data was achieved using the Array Assist 4.1 software (Stratagene). Normalization was performed using the GC-RMA algorithm.
|
| |
|
| Submission date |
Mar 02, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Genevieve PIETU |
| E-mail(s) |
gpietu@istem.genethon.fr
|
| Phone |
33169471154
|
| Fax |
33169471153
|
| Organization name |
INSERM
|
| Department |
U861
|
| Lab |
ISTEM
|
| Street address |
1 rue de l'Internationale
|
| City |
Evry |
| ZIP/Postal code |
91000 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE7179 |
Comparison of gene expression data between wild-type and DM1-affected undifferentiated hES cells. |
| GSE7214 |
Comparison of gene expression data between wild-type and DM1-affected cells |
|
| Relations |
| Reanalyzed by |
GSE119087 |
