|
| Status |
Public on Jul 01, 2015 |
| Title |
NY [mRNA] |
| Sample type |
RNA |
| |
|
| Source name |
Human Osteosarcoma Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NY
cell type: osteosarcoma cells
|
| Treatment protocol |
Cultured Cells are removed by treatment with Trypsin-EDTA when they reach to 70–80% confluence.
|
| Growth protocol |
Osteosarcoma cells were cultured in a high-glucose medium, DMEM supplemented with 10% FBS and 1% penicillin and streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the TRizol reagent and cDNA was synthesized according to the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was synthesized from total RNA using the Affymetrix 3` IVT Express Kit according to manufacturer’s protocols.
|
| |
|
| Hybridization protocol |
The double-strand cDNA was used as a template for in vitro transcription to generate biotin-labeled cRNA. After fragmentation, 12.5 ug of cRNA was hybridized to the GeneChip array for 16 hr.
|
| Scan protocol |
The GeneChips were washed and stained using the GeneChip Fluidics Station 450 (Affymetrix) and then scanned with the GeneChip Scanner 3000 (Affymetrix).
|
| Description |
Gene expression data from human osteosarcoma cell line
|
| Data processing |
The microarray numerical values were analyzed using the GeneSpring GX 11.0 software, according to the RMA16 Algorithm: quantile normalization, filter by flags (detected), filter by expression on the normalized data (20.0–100.0th percentile).
|
| |
|
| Submission date |
Jun 30, 2015 |
| Last update date |
Jul 01, 2015 |
| Contact name |
Masanori Kawano |
| E-mail(s) |
kawanom@hotmail.co.jp
|
| Phone |
+81-97-586-5872
|
| Organization name |
Oita University
|
| Street address |
1-1 Idaigaoka, Hasama-machi
|
| City |
Yufu |
| ZIP/Postal code |
879-5593 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE70414 |
mRNA Expression data from human osteosarcoma (OS) |
| GSE70415 |
mRNA and miRNA expression data from human osteosarcoma (OS) |
|
