|
| Status |
Public on Oct 01, 2015 |
| Title |
140156A_NM76 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood_never-smoker
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
age: 27y
smoking status: never-smoker
tissue: peripheral blood
|
| Treatment protocol |
4 mL peripheral venous fasting blood was collected from each subject in EDTA anticoagulant tube. The blood samples were maintained at room temperature for 30 minutes and then centrifuged at 1500g for 15min at 4℃. Then plasma was transferred to another clean EP tube and stored at – 80 ℃ until RNA extraction. Then, the total RNA was extracted from the plasma using the miRNeasy Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol. The quality and quantity of RNA were measured with a NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).plasma miRNA expression profiles were determined using the human microRNA array version 19.0 (Agilent, USA). Plasma miRNA from 28 present smokers and 12 never -smokers were detected with the miRNA array respectively.
|
| Growth protocol |
All subjects are from the same unit , do the same job, and live in the same community .
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using Qiagen miRNeasy Mini kit following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Labeled RNAs were heat denatured and hybridized to Agilent miRNA microarray, according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
According to the Agilent miRNA Microarray Protocol (Agilent Technologies).
|
| Scan protocol |
After hybridization and post-hybridization washes, slides were scanned immediately in Agilent Microarray Scanner with Surescan High Resolution Technology (Agilent Technologies, Santa Clara, CA).
|
| Description |
Gene expression in never-smoker plasma
|
| Data processing |
Feature Extraction v10.7.3.1 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and Bioconductor software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Jun 17, 2015 |
| Last update date |
Oct 01, 2015 |
| Contact name |
zhang suwei |
| E-mail(s) |
suweizhang@capitalbio.com
|
| Phone |
01080729390
|
| Organization name |
capitalbio
|
| Street address |
changping
|
| City |
beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platform ID |
GPL18402 |
| Series (1) |
| GSE69960 |
Cigarette smoking alters plasma microRNA expression profile in healthy young male smokers |
|
