|
| Status |
Public on Sep 08, 2016 |
| Title |
YUGASP NS shRNA replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
YUGASP cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: YUGASP
expression: non-silencing shRNA
|
| Treatment protocol |
YUGASP cells that either expressed Non-silencing shRNA (NS shRNA) or shRNAs targetingIFI6 (IFI6 shRNA #1 and IFI6 shRNA #2 were used for preparing the total RNA and to perfrom gene expression array.
|
| Growth protocol |
YUGASP NS shRNA,YUGASP PEA15 shRNA#1 and YUGASP PEA15 shRNA #2 cell lines were grown in DEMEM media with 10% Fetal bovine serum and Penicilline and Streptomycin at 37 C under 5% CO2 condition
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent (Invitrogen) as per the instruction from the manufacture and then purified using RNAeasy mini columns (Qiagen) as per the manufactures instruction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Signal intensities of probes were log2-transformed and Quantile normalized (lumi package in R/Bioconductor)
|
| |
|
| Submission date |
Jun 16, 2015 |
| Last update date |
Sep 08, 2016 |
| Contact name |
Narendra Wajapeyee |
| E-mail(s) |
nwajapey@uab.edu
|
| Phone |
205-934-5331
|
| Organization name |
University of Alabama at Birmingham
|
| Department |
Department of Biochemistry and Molecular Genetics
|
| Street address |
720 20th Street South, Kaul 540A
|
| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35233 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE69933 |
Regulators of NRAS-mediated transformation and melanoma tumor maintenance |
|
