|
| Status |
Public on Jun 01, 2016 |
| Title |
BB10a |
| Sample type |
RNA |
| |
|
| Source name |
Cells with high bone forming capacity in vivo
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Bone marrow derived stromal cells immortalized with telomerase gene
treatment: No treatment basal culture condition
passage: P83
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was isolated using the RNeasy Mini Kit (Qiagen), including RNase-free Dnase (Qiagen) digestion (RNase-free DNase Set, Quiagen) has been applied on the columns during isolation.
|
| Label |
biotin
|
| Label protocol |
Illumina Gene Expression protocols contain a first- and second-strand reverse transcription step, followed by a single in vitro transcription (IVT) amplification that incorporates biotin-labeled nucleotides. Subsequent steps include array hybridization, washing, blocking, and streptavadin-Cy3 staining. Fluorescence emission by Cy3 is quantitatively detected for downstream analysis.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalised using rank invariant normalisation after background subtraction using the Gene Expression Module 1.8.0 of GenomeStudio V2010.3 (Illumina).
|
| |
|
| Submission date |
May 29, 2015 |
| Last update date |
Jun 01, 2016 |
| Contact name |
Wasco Wruck |
| E-mail(s) |
wasco.wruck@med.uni-duesseldorf.de
|
| Organization name |
Universitätsklinikum Düsseldorf
|
| Street address |
Moorenstr. 5
|
| City |
Düsseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE69358 |
Association between in vivo bone formation and ex vivo migration capacity of human bone marrow stromal cells |
|
