|
| Status |
Public on Sep 01, 2016 |
| Title |
30_RPE_3d_NPAT_2 |
| Sample type |
RNA |
| |
|
| Source name |
hTERT-RPE1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: hTERT-RPE1
sirna: NPAT
day after sirna treatment: Day 3
replica: biological rep 2
|
| Treatment protocol |
For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
|
| Growth protocol |
cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified from cells using RNeasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
biotin
|
| |
|
| Hybridization protocol |
Standard Affymetrix
|
| Scan protocol |
Standard Affymetrix
|
| Description |
Gene expression data on 3d day after NPAT siRNA transfection
|
| Data processing |
The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
|
| |
|
| Submission date |
May 21, 2015 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Maria Sokolova |
| E-mail(s) |
maria.fulop@helsinki.fi
|
| Organization name |
University of Helsinki
|
| Department |
Institute of Biotechnology
|
| Lab |
Nuclear Actin Lab
|
| Street address |
Viikinkaari 5
|
| City |
Helsinki |
| ZIP/Postal code |
00014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL20234 |
| Series (2) |
| GSE69148 |
Differential response of normal and tumor cells to nucleosome depletion |
| GSE69149 |
Histone gene regulation in normal and tumor cells |
|
