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Sample GSM1693941 Query DataSets for GSM1693941
Status Public on Sep 01, 2016
Title 30_RPE_3d_NPAT_2
Sample type RNA
Source name hTERT-RPE1
Organism Homo sapiens
Characteristics cell line: hTERT-RPE1
sirna: NPAT
day after sirna treatment: Day 3
replica: biological rep 2
Treatment protocol For reverse transfection protocol, cells at 30% confluence were plated in the growth medium into 6-well plates using 15 nM siRNA and 7 µl HiPerFect (Qiagen) in final total volume 2 100 µl.
Growth protocol cells were cultured in media supplemented with penicillin/streptomycin and 10% FBS under standard culture conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified from cells using RNeasy kit (Qiagen)
Label biotin
Label protocol biotin
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Gene expression data on 3d day after NPAT siRNA transfection
Data processing The data were analyzed with RMA method in R. Probe set data extracted from custom CDF (hugene11sthsensgcdf_18.0.0)
Submission date May 21, 2015
Last update date Sep 01, 2016
Contact name Maria Sokolova
Organization name University of Helsinki
Department Institute of Biotechnology
Lab Nuclear Actin Lab
Street address Viikinkaari 5
City Helsinki
ZIP/Postal code 00014
Country Finland
Platform ID GPL20234
Series (2)
GSE69148 Differential response of normal and tumor cells to nucleosome depletion
GSE69149 Histone gene regulation in normal and tumor cells

Data table header descriptions
VALUE log2 RMA signal

Data table
ENSG00000000003_at 8.594520831
ENSG00000000005_at 3.49473489
ENSG00000000419_at 10.17003822
ENSG00000000457_at 6.597565677
ENSG00000000460_at 7.613120741
ENSG00000000938_at 4.80187036
ENSG00000000971_at 8.093063931
ENSG00000001036_at 9.922815895
ENSG00000001084_at 7.459255116
ENSG00000001167_at 8.524598162
ENSG00000001460_at 8.289882479
ENSG00000001461_at 8.514469873
ENSG00000001497_at 8.470120579
ENSG00000001561_at 4.2714149
ENSG00000001617_at 8.008770454
ENSG00000001626_at 3.167961511
ENSG00000001629_at 10.03510427
ENSG00000001631_at 7.893866742
ENSG00000002016_at 6.459325198
ENSG00000002079_at 5.338186301

Total number of rows: 22069

Table truncated, full table size 665 Kbytes.

Supplementary file Size Download File type/resource
GSM1693941_30_RPE_NPAT_2_D09.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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