|
| Status |
Public on Oct 03, 2016 |
| Title |
Day0WTATII |
| Sample type |
RNA |
| |
|
| Source name |
WT
|
| Organism |
Mus musculus |
| Characteristics |
cell type: AEC2
genotype/variation: wild type
treatment: untreated
|
| Growth protocol |
AEC2 cells were flow sorted from either untreated lungs or the lungs 4 days after bleomycin. The AEC2 cells were pooled from 4 – 5 mouse lungs each category.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
cDNA were amplyfied by Ovation Pico WT Amp V2 kit and labeling were done by the Encore Biotin Module (NuGen, San Carlos, CA)
|
| |
|
| Hybridization protocol |
Hybridization targets were prepared with the Ovation Pico WT Amp V2 kit (NuGen, San Carlos, CA) and the Encore Biotin Module (NuGen, San Carlos, CA) from total RNA, hybridized to GeneChip® mouse 430.2 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Command Console Scan Control 3.2.3.1515
|
| Description |
Gene expression data from WT D0 ATII
|
| Data processing |
GeneSpring GX
|
| |
|
| Submission date |
May 08, 2015 |
| Last update date |
Oct 03, 2016 |
| Contact name |
ting Xie |
| Organization name |
Cedars-Sinai Medical Center
|
| Department |
Pulmonary
|
| Street address |
127 San Vicente Blvd
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90034 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE68704 |
Hyaluronan and TLR4 Regulate Alveolar Stem Cell Renewal and Prevent Severe Pulmonary Fibrosis |
|
