|
| Status |
Public on Sep 14, 2015 |
| Title |
Tumor biopsy (P-421) |
| Sample type |
genomic |
| |
|
| Source name |
Cervical tumor
|
| Organism |
Homo sapiens |
| Characteristics |
figo stage: 1B2
histology: Squamous cell carcinoma
cohort: cohort 2
|
| Treatment protocol |
The samples were immediately snap-frozen in liquid nitrogen and stored at -80 C until used for analysis.
|
| Growth protocol |
1-4 tumor samples wer collected from each patient at the time of diagnosis
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted according to a standard protocol with proteinase K, phenol, chloroform and isoamylalcohol (cohort 1) or by the use of Purelink Genomic DNA Mini Kit (cohort 2)
|
| Label |
biotin and DNP
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Data processing |
Data processing was performed using the pipeline developed by Touleimat and Tost 2012.
|
| |
|
| Submission date |
May 05, 2015 |
| Last update date |
Sep 14, 2015 |
| Contact name |
Heidi Lyng |
| E-mail(s) |
heidi.lyng@rr-research.no
|
| Phone |
4722781478
|
| Organization name |
Oslo University Hospital
|
| Department |
Department of Radiation Biology
|
| Street address |
Montebello
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE68339 |
Interplay between promoter methylation and chromosomal loss in gene silencing at 3p11-p14 in cervical cancer |
|
