|
| Status |
Public on Oct 15, 2015 |
| Title |
Control rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Daphnia pulex neonates <96hours
|
| Organism |
Daphnia pulex |
| Characteristics |
developmental stage: neonate
stress: Control
dose: 0 ppb (control)
tissue: whole organsim
|
| Treatment protocol |
Fifty D. pulex were randomly selected from each replicate for small RNA extraction
|
| Growth protocol |
D. pulex were cultured in moderatly reconstituted hard water
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small RNAs including miRNAs were extracted by PureLink miRNA extraction kit by invitrogen.
|
| Label |
biotin
|
| Label protocol |
In brief, 300ng of small RNA from each sample was labeled using the Affymetrix FlashTag Bioton HSR kit.
|
| |
|
| Hybridization protocol |
The labeled samples were hybridized to the arrays for 17 hours and were stained and washed using the standard miRNA protocol.
|
| Scan protocol |
Affymetrix GeneChip Command Console Software was used to scan the arrays and generate CEL files.
|
| Description |
Gene expression data from Control
small RNAs
|
| Data processing |
Arrays were visually scanned for abnormalities or defects. CEL files were imported into Genespring 12.0 (Agilent, Santa Clara, CA, USA) to generate RMA (robust multi-array average) expression levels (Lrizarry et al., 2003) using the RMA normalization option.
|
| |
|
| Submission date |
May 03, 2015 |
| Last update date |
Oct 15, 2015 |
| Contact name |
SHUAI CHEN |
| E-mail(s) |
shuai@PURDUE.EDU
|
| Phone |
7653373586
|
| Organization name |
PURDUE UNIVERSITY
|
| Department |
Forestry and Natural Resources
|
| Lab |
105
|
| Street address |
195 Marsteller Street
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907 |
| Country |
USA |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE68481 |
Daphnia miRNA expression data from Cadmium exposed and none-exposed samples |
|
