|
| Status |
Public on Sep 16, 2015 |
| Title |
SLM2 plx rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
SLM2 plx
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SLM2
braf status: WT/WT
|
| Treatment protocol |
Cells were plated in RPMI + 5% FBS and incubated overnight before being treated with 125 nM plx4720 in phenol red–free RPMI-1640 without FBS for 8 hours at 37°C.
|
| Growth protocol |
Cells are grown in RPMI + 10% FBS and passaged at intervals of 80% confluence.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Qiashredder (Qiagen) and RNeasy Mini Kit (Qiagen). RNA was quantified on the NanoDrop 2000 spectrophotometer (Thermo Scientific) and RNA quality was inspected on a 1% agarose gel.
|
| Label |
Cy3
|
| Label protocol |
Biotin labeled RNA was hybridized to Illumina 3′IVT human HT-12 BeadChip arrays.
|
| |
|
| Hybridization protocol |
standard as recommended by illumina
|
| Scan protocol |
standard as recommended by illumina
|
| Description |
Replicate 1
|
| Data processing |
VSN By R package lumi
|
| |
|
| Submission date |
Apr 30, 2015 |
| Last update date |
Sep 16, 2015 |
| Contact name |
Stefan Bekiranov |
| E-mail(s) |
sb3de@virginia.edu
|
| Organization name |
University of Virginia
|
| Department |
Biochemistry and Molecular Genetics
|
| Lab |
Bekiranov Lab
|
| Street address |
1340 Jefferson Park Avenue
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22903 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE68453 |
Systems analysis of adaptive responses to MAP Kinase pathway blockade in BRAF mutant melanoma. |
|
