|
| Status |
Public on Sep 14, 2015 |
| Title |
Tumor biopsy [P-471] |
| Sample type |
RNA |
| |
|
| Source name |
Cervical tumor
|
| Organism |
Homo sapiens |
| Characteristics |
figo stage: 2B
histology: Squamous cell carcinoma
cohort: cohort 2
|
| Treatment protocol |
The samples were immediately snap-frozen in liquid nitrogen and stored at -80 C until used for analysis.
|
| Growth protocol |
1-4 tumor samples wer collected from each patient at the time of diagnosis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
In cohort 1, RNA was extracted with Trizol reagent, followed by double precipitation with isopropanol and final precipitation with 5M lithium chloride. In cohort 2, RNA was extracted by use of the miRNeasy mini kit. RNA from different biopsies of the same tumor was pooled. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
P-471
|
| Data processing |
The data were normalised using quantile normalisation in BeadStudio 3.1.3.0
|
| |
|
| Submission date |
Apr 28, 2015 |
| Last update date |
Sep 14, 2015 |
| Contact name |
Heidi Lyng |
| E-mail(s) |
heidi.lyng@rr-research.no
|
| Phone |
4722781478
|
| Organization name |
Oslo University Hospital
|
| Department |
Department of Radiation Biology
|
| Street address |
Montebello
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE68339 |
Interplay between promoter methylation and chromosomal loss in gene silencing at 3p11-p14 in cervical cancer |
|
| Relations |
| Reanalyzed by |
GSM1869070 |
