|
| Status |
Public on Sep 30, 2015 |
| Title |
Dox(-) - siControl |
| Sample type |
RNA |
| |
|
| Source name |
HeLa-shDicer cells treated without Dox
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa cells inducibly expressing shDicer under control of Doxycycline (HeLa-shDicer cells)
agent: no Dox
genotype/variation: siControl
|
| Treatment protocol |
The cells were transfected with siControl or siAgp2. After 48 h incubation, total RNA was extracted using Isogen (Nippongen).
|
| Growth protocol |
Hela-shDicer cells, which inducibly express shDicer by Dox treatment, were cultured in Dox-containing medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isogen extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated samples were prepared using FlashTag Biotin HSR RNA labeling Kit for Affymetrix GeneChip miRNA Arrays according the the user manual.
|
| |
|
| Hybridization protocol |
The samples were hybridized for 18 hr on GeneChip miRNA 4.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
The normalization of RMA was performed using Affymetrix Expression Console Software.
|
| |
|
| Submission date |
Apr 17, 2015 |
| Last update date |
Sep 30, 2015 |
| Contact name |
Mitsuhiro Machitani |
| E-mail(s) |
mmachita@ncc.go.jp
|
| Phone |
081-3-3547-5201
|
| Organization name |
National Cancer Center Research Institute
|
| Lab |
Division of Cancer Stem Cell
|
| Street address |
5-1-1 Tsukiji
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE67992 |
Expression data from Dicer and Ago2-knockdown HeLa cells |
|
