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Sample GSM1644

Query DataSets for GSM1644

Status

Public on May 01, 2002

Title

BYC-BY

Sample type

RNA

Channel 1

Source name

independent culture of BY4716

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from the lab strain BY4716 (isogenic to S288c).
See Brem et al., Science 296(5568):752-755.
Lot batch =

Submission date

Apr 26, 2002

Last update date

May 28, 2005

Contact name

Rachel Brem

E-mail(s)

rbrem@fhcrc.org

Phone

206-667-5659

Fax

206-667-5978

URL

http://www.fhcrc.org/labs/kruglyak/index.html

Organization name

Fred Hutchinson Cancer Research Institute

Department

Human Biology

Lab

Kruglyak

Street address

1100 Fairview Ave. N. D4-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE38

BY_wild_parents

Data table header descriptions
ID_REF	Spot number from platform GPL118
CH1_MEAN	Median signal at 635 nm
CH1_BKD_MEAN	Median background signal at 635 nm
CH2_MEAN	Median signal at 532 nm
CH2_BKD_MEAN	Median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	862	87	841	180	0.036859	0
1	487	89	535	174	0.125636	0
2	463	94	506	169	0.135533	0
3	502	103	503	172	-0.003152	0
4	365	109	427	171	0.266406	0
5	687	118	603	179	-0.157959	0
6	923	97	725	170	-0.307248	0
7	787	104	716	169	-0.053939	0
8	1195	119	769	180	-0.602933	0
9	609	104	627	168	0.128616	0
10	845	111	830	174	0.104321	0
11	5507	121	4106	182	-0.190484	0
12	4539	125	2605	182	-0.598887	0
13	1668	120	1306	176	-0.187677	0
14	588	116	578	172	0.049098	0
15	2376	134	1772	189	-0.235720	0
16	3371	134	2488	188	-0.226618	0
17	2049	114	2030	168	0.210925	0
18	1056	124	1009	189	0.081699	0
19	9218	140	10728	196	0.480739	0

Total number of rows: 7085

Table truncated, full table size 221 Kbytes.

Supplementary data files not provided