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Sample GSM1641 Query DataSets for GSM1641
Status Public on May 01, 2002
Title BY-RM2
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name independent culture of wild strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from the lab strain BY4716 (isogenic to S288c)
and a wild isolate strain from a California vineyard
collected by R. Mortimer. See Brem et al., Science
296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE38 BY_wild_parents

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 460 70 630 124 -0.333207 0
1 270 71 378 121 -0.326544 0
2 227 92 397 141 -0.880728 0
3 229 70 482 118 -1.152456 0
4 162 71 286 117 -0.850629 0
5 319 73 403 115 -0.184955 0
6 363 72 445 113 -0.147708 0
7 381 72 501 113 -0.285994 0
8 355 70 481 112 -0.330203 0
9 300 73 344 112 0.011023 0
10 342 73 441 110 -0.256769 0
11 2028 75 1473 110 0.561362 0
12 1458 76 2737 110 -0.884203 0
13 668 77 739 111 -0.045150 0
14 259 77 392 109 -0.594408 0
15 846 76 859 108 0.078502 0
16 1175 78 1185 110 0.071683 0
17 969 79 896 109 0.219898 0
18 492 78 472 109 0.232117 0
19 11845 81 2439 108 2.377814 0

Total number of rows: 7085

Table truncated, full table size 210 Kbytes.




Supplementary data files not provided
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