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Sample GSM1638 Query DataSets for GSM1638
Status Public on May 01, 2002
Title BYB-BY
Sample type RNA
 
Channel 1
Source name independent culture of BY4716
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from the lab strain BY4716 (isogenic to S288c).
See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE38 BY_wild_parents

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 2045 89 2611 159 0.469768 0
1 1406 88 1569 150 0.250240 0
2 1308 88 1310 147 0.074685 0
3 1438 90 1318 149 -0.061830 0
4 764 104 786 161 0.065106 0
5 1774 103 1790 158 0.109645 0
6 1160 86 1247 139 0.188679 0
7 1817 87 1903 135 0.175062 0
8 2761 87 2122 135 -0.284692 0
9 877 82 1015 131 0.296807 0
10 1817 92 1990 144 0.241522 0
11 9756 113 8594 162 -0.049892 0
12 8717 126 5396 170 -0.573403 0
13 3627 110 3336 160 -0.003419 0
14 1157 97 1183 142 0.117621 0
15 6044 103 5072 145 -0.126281 0
16 5807 107 5355 152 0.012097 0
17 3239 107 3625 154 0.291983 0
18 2505 130 2610 180 0.176744 0
19 12829 124 12066 168 0.049038 0

Total number of rows: 7085

Table truncated, full table size 217 Kbytes.




Supplementary data files not provided
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