|
| Status |
Public on Apr 29, 2015 |
| Title |
RNA-Seq minus BIC |
| Sample type |
SRA |
| |
|
| Source name |
cortical neurons
|
| Organism |
Mus musculus |
| Characteristics |
cell type: primary cortical neurons
|
| Treatment protocol |
Cortical neurons were treated with 10nM purified recombinant Reelin for 1 hour.
|
| Growth protocol |
Cortical neurons were dissected from E15.5 embryos and cultured for 10-11 DIV.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was exctracted with the Qiagen RNAeasy kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
RNA-Seq in primary cortical neurons in restin condition
|
| Data processing |
Basecalls performed using CASAVA version 1.4
RNA-seq reads were aligned to the mm9 genome assembly using Bowtie version 2.0.1.
The bigwig files were generated by using Homer v3.9, which the total tags are normalized to 1.00e+07.
Genome_build: mm9
Supplementary_files_format_and_content: bigWig
|
| |
|
| Submission date |
Mar 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Qi Ma |
| E-mail(s) |
q1ma@ucsd.edu
|
| Organization name |
UCSD
|
| Street address |
9500 Gilman Dr,
|
| City |
San Diego |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE66706 |
LRP8-Reelin-regulated Neuronal (LRN) Enhancer signature underlying learning and memory formation (RNA-Seq 1) |
| GSE66710 |
LRP8-Reelin-regulated Neuronal (LRN) Enhancer signature underlying learning and memory formation |
|
| Relations |
| BioSample |
SAMN03396330 |
| SRA |
SRX914978 |
