NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1629 Query DataSets for GSM1629
Status Public on May 01, 2002
Title BY-BY1
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name independent culture of BY4716
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from the lab strain BY4716 (isogenic to S288c).
See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE38 BY_wild_parents

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1581 62 1375 128 0.161970 0
1 971 63 849 129 0.212005 0
2 703 69 773 135 -0.131764 0
3 806 67 963 130 -0.295433 0
4 313 62 384 123 -0.179053 0
5 1084 63 977 119 0.128243 0
6 313 62 352 117 -50
7 1495 63 1358 120 0.087330 0
8 1536 66 1283 120 0.215274 0
9 561 63 571 114 0.001261 0
10 1260 62 1263 113 -0.063697 0
11 5769 62 5191 116 0.046634 0
12 2795 63 4660 117 -0.856378 0
13 1972 63 1862 116 0.006073 0
14 674 63 756 115 -0.191843 0
15 2789 64 2626 115 -0.004696 0
16 3558 65 3236 116 0.040230 0
17 1980 65 1760 115 0.096566 0
18 1170 68 941 118 0.298469 0
19 8023 71 7737 118 -0.060975 0

Total number of rows: 7085

Table truncated, full table size 213 Kbytes.




Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap