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Sample GSM1627 Query DataSets for GSM1627
Status Public on May 01, 2002
Title BY-7.6.c
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 725 70 618 58 0.078180 0
1 384 74 307 54 0.145243 0
2 360 72 205 49 0.736635 0
3 314 68 297 48 -0.165375 0
4 230 68 176 48 0.191962 0
5 468 72 342 49 0.286712 0
6 228 66 186 49 0.093930 0
7 555 65 410 48 0.288904 0
8 586 66 437 48 0.270854 0
9 415 64 282 48 0.437075 0
10 553 65 400 49 0.327522 0
11 3385 68 2902 50 0.070017 0
12 2318 68 837 51 1.369436 0
13 1067 66 796 49 0.274374 0
14 346 67 289 49 0.069343 0
15 1342 68 1244 51 -0.053117 0
16 1977 71 1659 54 0.100087 0
17 1693 73 1135 53 0.434406 0
18 789 71 554 51 0.365538 0
19 8350 70 4147 50 0.867175 0

Total number of rows: 7085

Table truncated, full table size 213 Kbytes.




Supplementary data files not provided
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