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Sample GSM1620 Query DataSets for GSM1620
Status Public on May 01, 2002
Title 7.4.c-BY
Sample type RNA
 
Channel 1
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 2257 83 2701 95 0.170926 0
1 1090 81 1566 86 0.462112 0
2 992 82 1359 85 0.394868 0
3 1614 80 1219 86 -0.527709 0
4 797 79 965 85 0.202961 0
5 1195 77 1275 85 -0.000517 0
6 417 75 439 88 -50
7 2216 80 2600 97 0.138188 0
8 1508 80 2378 86 0.592052 0
9 1184 78 1775 93 0.514268 0
10 1778 83 2371 102 0.330213 0
11 7708 96 9082 109 0.146756 0
12 12239 102 4678 111 -1.500652 0
13 4198 95 4439 104 -0.011206 0
14 1308 92 1446 97 0.059188 0
15 5800 89 6672 98 0.112469 0
16 6209 92 7578 103 0.198691 0
17 5541 98 6714 116 0.187068 0
18 2316 104 3142 132 0.353853 0
19 6172 99 10163 119 0.635294 0

Total number of rows: 7085

Table truncated, full table size 216 Kbytes.




Supplementary data files not provided
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