Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix GeneChip Expression Analysis Technical Manual, Rev5, using 5 ug total RNA
Hybridization protocol
standard Affymetrix GeneChip Expression Analysis Technical Manual, Rev5
Scan protocol
Scanning was performed using the Affymetrix Fluidic Station 450, protocol EuKGE-WS2v5
Description
DM2 patient, age 47, gender Male
Data processing
from the CEL files, we normalyzed and analyzed the data using the DChip 2006 (DNA-Chip Analyzer) software (MBEI analysis, PM only model). Build date: Feb 16 2006. Normalization was carried against a normal control sample (N_M43_?_03)
