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Sample GSM1617 Query DataSets for GSM1617
Status Public on May 01, 2002
Title BY-7.1.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1007 65 963 66 0.027787 0
1 543 65 524 64 0.012544 0
2 553 67 489 62 0.143888 0
3 495 65 544 62 -0.207529 0
4 317 66 328 63 -0.121137 0
5 708 66 645 62 0.096245 0
6 324 66 314 61 -0.014599 0
7 762 67 761 62 -0.051112 0
8 748 65 713 62 0.026396 0
9 557 65 527 61 0.035496 0
10 833 65 839 63 -0.057783 0
11 4940 66 4063 65 0.242995 0
12 3204 70 6213 67 -1.014475 0
13 1411 70 1380 68 -0.011291 0
14 526 69 501 64 0.021728 0
15 2181 68 2138 64 -0.015956 0
16 2868 67 2628 63 0.084151 0
17 2057 68 1639 63 0.292943 0
18 1174 68 946 62 0.280401 0
19 13044 72 7296 64 0.800102 0

Total number of rows: 7085

Table truncated, full table size 206 Kbytes.




Supplementary data files not provided
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