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Sample GSM1615 Query DataSets for GSM1615
Status Public on May 01, 2002
Title BY-6.6.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 909 56 733 73 0.201143 0
1 487 60 380 69 0.288384 0
2 506 63 330 71 0.605418 0
3 472 61 491 75 -0.186382 0
4 292 58 278 75 0.036092 0
5 660 59 478 70 0.389859 0
6 479 57 408 70 0.151283 0
7 846 59 704 71 0.145221 0
8 842 60 751 71 0.032697 0
9 688 58 596 70 0.091352 0
10 805 58 587 70 0.362007 0
11 4970 58 3040 70 0.556911 0
12 3033 59 4986 71 -0.893724 0
13 1559 60 1183 71 0.261907 0
14 519 59 469 70 0.036308 0
15 2054 65 1523 76 0.290041 0
16 2762 69 2121 78 0.229588 0
17 2344 60 1419 70 0.590735 0
18 1017 60 635 69 0.588780 0
19 10647 62 4698 70 1.024623 0

Total number of rows: 7085

Table truncated, full table size 206 Kbytes.




Supplementary data files not provided
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