NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1614 Query DataSets for GSM1614
Status Public on May 01, 2002
Title BY-6.5.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1656 65 2042 71 -0.100942 0
1 999 60 877 68 0.423038 0
2 1007 62 1478 68 -0.369257 0
3 910 64 1840 69 -0.857782 0
4 411 64 665 69 -0.572324 0
5 1300 64 1283 68 0.232775 0
6 638 63 747 69 -0.029671 0
7 1553 64 1625 68 0.143627 0
8 1680 65 1404 67 0.480587 0
9 1065 65 1081 67 0.187995 0
10 1346 67 1474 72 0.075582 0
11 8390 68 6009 73 0.695491 0
12 3493 69 16915 73 -2.090257 0
13 2963 69 3303 73 0.049583 0
14 907 71 1392 72 -0.450911 0
15 4234 74 4859 72 0.005514 0
16 5267 76 5713 76 0.089137 0
17 3828 77 3479 77 0.348944 0
18 1956 78 1408 72 0.699329 0
19 12621 75 7368 72 0.990102 0

Total number of rows: 7085

Table truncated, full table size 209 Kbytes.




Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap