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Sample GSM1606 Query DataSets for GSM1606
Status Public on May 01, 2002
Title BY-6.2.b
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 2695 95 3333 135 0.067259 0
1 1410 88 1506 134 0.312359 0
2 1133 92 1368 134 0.120545 0
3 1109 85 1621 126 -0.180013 0
4 748 83 932 123 0.083132 0
5 1464 83 1700 123 0.174448 0
6 1495 87 1686 132 0.223578 0
7 2202 103 2342 145 0.300085 0
8 1887 96 2629 141 -0.108304 0
9 1616 89 1532 132 0.491190 0
10 1105 96 1157 134 0.346037 0
11 7065 116 4907 151 0.912974 0
12 6317 137 13450 184 -0.736137 0
13 3937 127 4070 175 0.334085 0
14 1294 117 1441 153 0.235899 0
15 5910 150 6574 175 0.214140 0
16 5641 146 5600 172 0.383616 0
17 7386 166 6168 188 0.637771 0
18 3054 170 2592 183 0.625554 0
19 11348 163 5926 169 1.324093 0

Total number of rows: 7085

Table truncated, full table size 222 Kbytes.




Supplementary data files not provided
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