|
Status |
Public on Feb 07, 2007 |
Title |
PCI 5002 Zinc A |
Sample type |
RNA |
|
|
Source name |
non-cycling plateau phase A549 lung cancer cell culture
|
Organism |
Homo sapiens |
Characteristics |
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Biomaterial provider |
ATCC
|
Treatment protocol |
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Growth protocol |
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Extracted molecule |
total RNA |
Extraction protocol |
We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
|
Label |
Affymetrix single-stage biotin
|
Label protocol |
We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
|
|
|
Hybridization protocol |
We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
|
Scan protocol |
We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
|
Description |
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Data processing |
RMA Normalization
|
|
|
Submission date |
Feb 06, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Joseph Gerard Hacia |
E-mail(s) |
hacia@hsc.usc.edu
|
Phone |
323-442-3030
|
Organization name |
University of Southern California
|
Department |
Biochemistry and Molecular Biology
|
Lab |
Hacia Lab
|
Street address |
2250 Alcazar Street, IGM 261
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90089 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE6960 |
Synthesis and Anticancer Properties of Water-Soluble Zinc Ionophores 1 |
GSE6972 |
Synthesis and Anticancer Properties of Water-Soluble Zinc Ionophore: Cell Culture and Xenograft Model |
|
Relations |
Reanalyzed by |
GSE64985 |
Reanalyzed by |
GSE119087 |