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Sample GSM1604 Query DataSets for GSM1604
Status Public on May 01, 2002
Title BY-5.5.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) rbrem@fhcrc.org
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1007 81 1364 75 -0.430367 0
1 553 78 548 72 0.043768 0
2 796 76 578 69 0.547133 0
3 736 76 1126 71 -0.629903 0
4 473 76 499 70 -0.065037 0
5 782 79 779 68 0.030477 0
6 260 78 247 68 -50
7 1010 75 1130 66 -0.139658 0
8 879 75 767 66 0.244583 0
9 535 76 460 66 0.267100 0
10 775 76 861 66 -0.138861 0
11 4801 78 4276 69 0.213713 0
12 3264 80 5026 71 -0.591243 0
13 1355 81 1305 68 0.089321 0
14 544 81 537 66 0.022087 0
15 1612 82 1765 72 -0.099249 0
16 2504 90 2452 80 0.072123 0
17 1175 93 1199 78 -0.004284 0
18 743 86 579 73 0.423558 0
19 2966 84 2818 71 0.116015 0

Total number of rows: 7085

Table truncated, full table size 211 Kbytes.




Supplementary data files not provided
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