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Sample GSM1603

Query DataSets for GSM1603

Status

Public on May 01, 2002

Title

6.4.d-BY

Sample type

RNA

Channel 1

Source name

segregant from BY4716 x wild wine strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Channel 2

Source name

reference pool of BY4716 strain

Organism

Saccharomyces cerevisiae

Extracted molecule

total RNA

Description

RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =

Submission date

Apr 26, 2002

Last update date

May 28, 2005

Contact name

Rachel Brem

E-mail(s)

rbrem@fhcrc.org

Phone

206-667-5659

Fax

206-667-5978

URL

http://www.fhcrc.org/labs/kruglyak/index.html

Organization name

Fred Hutchinson Cancer Research Institute

Department

Human Biology

Lab

Kruglyak

Street address

1100 Fairview Ave. N. D4-100

City

Seattle

State/province

ZIP/Postal code

98109

Country

USA

Platform ID

GPL118

Series (1)

GSE37

BYxwild_40

Data table header descriptions
ID_REF	Spot number from platform GPL118
CH1_MEAN	Median signal at 635 nm
CH1_BKD_MEAN	Median background signal at 635 nm
CH2_MEAN	Median signal at 532 nm
CH2_BKD_MEAN	Median background signal at 532 nm
VALUE	First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG	GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF	CH1_MEAN	CH1_BKD_MEAN	CH2_MEAN	CH2_BKD_MEAN	VALUE	FLAG
0	1473	69	2384	84	0.289825	0
1	539	63	1219	76	0.841526	0
2	1040	65	1020	81	-0.476543	0
3	1090	72	1276	86	-0.197042	0
4	508	69	817	83	0.319293	0
5	1011	68	1662	84	0.320501	0
6	231	69	326	84		-50
7	1344	68	2207	86	0.310850	0
8	1186	72	2302	91	0.566684	0
9	949	72	1870	91	0.598152	0
10	987	73	2012	93	0.647823	0
11	3578	78	7303	99	0.619177	0
12	6532	86	4797	112	-0.882621	0
13	2604	92	4215	121	0.282409	0
14	1026	89	1398	115	0.031134	0
15	3104	83	5179	118	0.322130	0
16	3753	84	6137	118	0.291871	0
17	3713	86	5779	121	0.219249	0
18	1099	77	2321	103	0.695598	0
19	7395	84	11374	114	0.200800	0

Total number of rows: 7085

Table truncated, full table size 213 Kbytes.

Supplementary data files not provided